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Triple-labeled fluorescence and AFM images of an Endothelial cell

Left: Fixed endothelial cells (HUVECC cell line) were labeled with Hoescht 33342 (nucleus), Alexa Fluor 488 (actin filaments) and Alexa Fluor 546 (ICAM-1) and viewed with wide-field fluorescence using the Nikon TE2000U inverted microscope and a 40x objective. Right: The AFM image was scanned at the area indicated by the box and then rendered in 3D, 35 ┬Ám scan. Alexa Fluor 546 and antibody courtesy of R. Waugh and E. Lomakina, University of Rochester.

Date: 16th November 17

Author: Asylum Research

Category: Asylum Gallery Image

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